Propagation 
Probably, the life of Okinawan Orchis are considered to be about 25 to 30
years. As for the aged clones, growth becomes bad, and the rate of propagation
also falls. In order to obtain a healthy individual, propagation by the seed is
required.
years. As for the aged clones, growth becomes bad, and the rate of propagation
also falls. In order to obtain a healthy individual, propagation by the seed is
required.
Orchid seeds are not like other seeds in that they are tiny (they are often
referred to as dust seeds) and contain few food reserves. In nature, they
usually will not grow unless infected by a particular fungus, which feeds the
young plants with all the sugars and nutrients they need until large enough.
referred to as dust seeds) and contain few food reserves. In nature, they
usually will not grow unless infected by a particular fungus, which feeds the
young plants with all the sugars and nutrients they need until large enough.
Seeds of Okinawan Orchis. 
compare with a Sesame.
Little research about Symbiotic fungus ('symbiotic' is the partner relationship
between fungus and orchid.) of this orchid has been done in Japan, so that
nobody knows for symbiotic germination.
between fungus and orchid.) of this orchid has been done in Japan, so that
nobody knows for symbiotic germination.
but this orchids that grows readily from seed under aseptic conditions. (that is,
non-symbiotic germination).
non-symbiotic germination).
In flask germination the seeds are germinated under sterile conditions on a
agar-based medium which contains all the sugars and minerals the seeds need
to germinate and grow. The medium, to a certain extent, mimic the nutrients
which would have been provided by the fungus and allow the seeds to germinate
and grow without it. (>>My culture medium)
to germinate and grow. The medium, to a certain extent, mimic the nutrients
which would have been provided by the fungus and allow the seeds to germinate
and grow without it. (>>My culture medium)
hand pollination : use a toothpick which made the tip sharp.
left : 7 days after hand pollination.
middle: 28 days after.
right : 70 days after, mature Capsule ( fruit of orchid) and Seeds.
left: Protocorm ( premature seedling of orchid ) with Root-hair.
right: Young seedlings
left: Seedlings in flask.
right: flower in flask.
left : New tubers in flask.
right: First flower is very small.
3 years, Mature plant.
